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浮游植物分類熒光儀——PHYTO-PAM-II
日期:2017-01-04 22:12:05

主要功能

●對(dui)自(zi)然水(shui)體(ti)中的藍藻、綠(lv)藻、硅/甲藻和(he)隱藻自(zi)動分類(定性)

●自(zi)動測量水樣中藍藻、綠藻、硅/甲藻和隱藻的葉(xie)綠素(su) a 含量(定(ding)量)和總葉(xie)綠素(su) a 含量

●一(yi)杯(bei)自然水樣,同時獲得藍藻(zao)(zao)(zao)(zao)、綠藻(zao)(zao)(zao)(zao)、硅/甲藻(zao)(zao)(zao)(zao)和隱藻(zao)(zao)(zao)(zao)的光合活性:

●光合效率(lv)和光合速率(lv)(相對(dui)電子傳遞速率(lv))

●快速(su)光曲線并進行擬合

●藻類的潛(qian)在最大光合(he)效率

●藻類的光保護能力

●藻類耐受(shou)強光的能力

●用戶可做自己(ji)的參考光譜


測量參數

Fo, Fm, F, Fm', Fv/Fm, Y(II) 即 ΔF/Fm', ETR, a, Ik, Pm, PAR 和葉綠素含量等

 

應用領域

●主要用于水生(sheng)(sheng)(sheng)生(sheng)(sheng)(sheng)物學、水域生(sheng)(sheng)(sheng)態(tai)學、海洋學、湖沼學、水質預(yu)警(jing)、微藻生(sheng)(sheng)(sheng)理學、微藻抗逆性等領域,對于了解自然水樣中藻類(lei)種群的動態(tai)變(bian)化、水華(hua)預(yu)警(jing)、野外水體(ti)中光合作用的時(shi)空(kong)變(bian)化、校正初級生(sheng)(sheng)(sheng)產力的計算(suan)等有較(jiao)大(da)幫助。

●特(te)別適于浮(fu)游植物(wu)動(dong)力(li)學研究和(he)有害水(shui)華的早期(qi)預警。

 

主要技術參數

●主控單元:金屬外(wai)殼,包含所有的光電元件及樣品測量室

●測(ce)(ce)量(liang)光(guang)(guang): LED,440 nm,480 nm,540 nm,590 nm 和(he) 625 nm,5 波長脈沖調(diao)制測(ce)(ce)量(liang)光(guang)(guang),2 檔強度設(she)置(zhi),8 檔調(diao)制頻率設(she)置(zhi), 3 檔測(ce)(ce)量(liang)光(guang)(guang)自動高(gao)頻設(she)置(zhi)

●光(guang)化光(guang):板(ban)載多波長(chang) LED 陣列,440 nm,480 nm,540 nm,590 nm,625 nm 和(he) 420-640 nm(白光(guang)),提供持續光(guang)化光(guang),最高(gao)可(ke)達(da) 1400 μmol m-2 s-1,快速動力學(xue)閃光(guang)高(gao)達(da) 7000 μmol m-2 s-1,飽(bao)和(he)脈沖最高(gao)可(ke)達(da) 5000 μmol m-2 s-1

●遠(yuan)紅光:725 nm LED

●信號(hao)檢(jian)測:基于 H-10720 光(guang)電(dian)傳(chuan)感器模塊的光(guang)電(dian)倍增(zeng)檢(jian)測器

●標準檢(jian)測(ce)過濾器:> 650 nm 長通濾光(guang)片

●高(gao)分辨(bian)率(lv)的(de)光電(dian)倍增(zeng)管,葉綠素(su)濃度(du)檢測限低至 0.1μg/L,適用野(ye)外(wai)采集的(de)藻濃度(du)很稀(xi)的(de)樣(yang)品(pin)

 

升級的技術特點

●可提供(gong) 5 種波長(chang)的脈(mo)沖調制測量光和光化光

●板載 LED 陣(zhen)列芯片技術

●可實時進行四種藻的分類

●可進(jin)行(xing)標準 PAM 測量及(ji)不同波長強光(guang)(guang)化光(guang)(guang)誘導的毫秒(miao)級熒光(guang)(guang)上升動力(li)學分析

●可測定光系統 II 功能性捕(bu)光截(jie)面積

●內置自動測量(liang)程序,易(yi)于(yu)操作

 

PHYTO-PAM-II vs PHYTO-PAM

  便攜式 PHYTO-PAM-II

  PHYTO-PAM

  5 種(zhong)不同波長的測(ce)量(liang)光用于(yu)生(sheng)物體內不同類型的天線色素熒光的激發(fa)

  4 種不(bu)同波長的(de)光

  4 種色素類型(xing)的在線分類

  3 種色素類型的在線(xian)分類

  6 種波(bo)長的光化光

  1 種(zhong)波長的光(guang)化(hua)光(guang)

  可以(yi)分別測量(liang)綠藻(zao)(zao),藍(lan)藻(zao)(zao),硅/甲藻(zao)(zao)以(yi)及(ji)含(han)有(you)(you)藻(zao)(zao)紅(hong)蛋白的有(you)(you)機體(ti),如(ru)隱藻(zao)(zao)不同波長下 PSII 的活性(xing)

  無該功能(neng)

  緊湊型設計

  需(xu)要組裝(zhuang)

  增加(jia)了(le)快速(su)動力學操作模式,可通過強光(guang)(guang)化光(guang)(guang)脈(mo)沖,測量(liang)不同波(bo)長(chang)的 O- I1 熒光(guang)(guang)上(shang)升動力學曲線

  無該(gai)功能

  通過測定(ding)不同光(guang)質和(he)光(guang)合生物色(se)素復合體,獲得光(guang)系(xi)統 II 功能(neng)性捕光(guang)截面積的(de)信息即(ji) σPSII

  無(wu)該功能

 ; 通過 FluoRed 熒(ying)光標準(zhun)將參考光譜校(xiao)準(zhun)標準(zhun)化

  無該功能

  獲得的參考(kao)光譜可在不同設備(bei)及用戶間互換使用

  參考光譜不能互(hu)換

  

選購指南

一、實驗室測量基本款

系統(tong)組成(cheng):實(shi)驗室版主機,激發檢測單元,懸浮液的光(guang)學單元,球(qiu)形光(guang)量子傳感器(qi),工作臺(tai),軟件(jian)等

注意:新(xin)版 PHYTO-PAM-II 必須配置光量(liang)子傳(chuan)感器,校準光強(qiang)后參考(kao)光譜可(ke)在不同(tong)儀器間通用

Phyto-PAM II laboratory version.jpg

實驗室測量基本款

  

二、野外便攜緊湊款

系(xi)統(tong)組(zu)成:緊(jin)湊型(xing)主(zhu)機,球(qiu)形光量子傳感器,軟件等(deng)

注意:新版 PHYTO-PAM-II 必須配置光量子傳感器,校準光強后參考光譜可在不同儀器間通用

Phytho-PAMM-II_071_1-small-2.jpg

便攜式緊湊款


Channels.jpg

algae.jpg

Reference.jpg

五種測量(liang)光通道

區分四種藻(zao)類

通用型參考光譜(pu)

軟件界面7.png

light-curve.jpg

Fast-Kinetics.jpg

測量慢速(su)動力學曲(qu)線

淬滅(mie)分析(xi),暗弛豫(yu)分析(xi)

測量快速(su)光曲線

光(guang)響應曲線(xian)

測量快速動力學曲(qu)線

分析PSII功能性捕光(guang)截面


三、其他可選附件

1,WATER-S:攪(jiao)拌(ban)器(推薦選配),利用內置(zhi)電池(chi)驅(qu)動的馬達帶動攪(jiao)拌(ban)棒旋轉,對樣品杯里的懸浮液進行(xing)攪(jiao)拌(ban)。需(xu)配置(zhi)攪(jiao)拌(ban)棒 WATER-R。

2,WATER-R:攪拌棒(標配10根(gen))

 

產地:德國WALZ


參考文獻

數據來(lai)源:光(guang)合作(zuo)用文(wen)(wen)獻 Endnote 數據庫,更新至 2021 年 1 月,文(wen)(wen)獻數量超過 10000 篇

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