簡(jian)介
OX1LP溶解(jie)氧(yang)測(ce)量儀(yi)使用(yong)極(ji)譜溶解(jie)氧(yang)電極(ji)，可用(yong)于測(ce)量水(shui)相(xiang)中的(de)光(guang)合(he)作用(yong)和(he)/或(huo)呼吸作用(yong)，適(shi)用(yong)于藻類(lei)、植物、動物和(he)細胞(bao)器(qi)（葉綠體和(he)線(xian)粒體）的(de)懸浮液。還可用(yong)于監測(ce)在水(shui)相(xiang)中產生或(huo)消耗氧(yang)氣(qi)的(de)化學(xue)和(he)生化反應。溶解(jie)氧(yang)包配有儀(yi)器(qi)、附件、軟件和(he)接口(kou)。
可提供從100ul（流通）到50ml不同體(ti)積(ji)的DO電極(ji)。水套反應杯內的氧電極(ji)可用于測(ce)量生物樣品(pin)或(huo)酶反應的O2消耗(hao)（或(huo)產生）。
采用(yong)簡(jian)單的兩點校準(zhun)， 可在(zai)相同(tong)的溫度下(xia)進行校準(zhun)和(he)實驗，或者可以在(zai)軟件(jian)(jian)中對不同(tong)溫度下(xia)的測量進行簡(jian)單的溫度校正(zheng)。隨機附校準(zhun)所需的所有(you)附件(jian)(jian)。
測量對(dui)象 v 藻類(lei)光(guang)合作用(yong)與呼吸作用(yong) v 細菌(jun)呼吸 v 微生物呼吸(xi) v 小型水生動物(wu)的呼吸 v 水生昆(kun)蟲和幼蟲呼吸 v 線(xian)粒體呼吸(xi) v 葉綠(lv)體O2的產生 v 細胞懸浮呼(hu)吸 v 植(zhi)物細(xi)胞懸(xuan)浮光(guang)合作用 | 應用領(ling)域 v 水生植(zhi)物(wu)光合氧代謝(xie)的測定(ding) v 抑制劑對線粒體電(dian)子傳遞的影響 v 光依(yi)賴性葉綠(lv)體電(dian)子輸運分析 v 溫(wen)度對水(shui)生動物(wu)攝氧量的影響 v 酶(mei)動力學耗氧研究 |
基(ji)本組件 溶解(jie)氧反應杯電極（1,2.5,4,6,30,50ml） 反應杯電極放大器(qi) 磁力攪拌器 攪拌(ban)棒和O形(xing)圈 KCl填充液 膜材料(liao)和(he)濾紙 水(shui)套用(yong)10英尺Excelon管 LabQuest迷你數據(ju)接口 Logger Pro軟(ruan)件 定制(zhi)安裝軟件 操作手冊(ce) 可(ke)選組件： 向反(fan)應杯中添加代謝物的注(zhu)射器； 10微(wei)(wei)升(sheng)，50微(wei)(wei)升(sheng)，100微(wei)(wei)升(sheng) 樣(yang)品溫度測量用熱敏電阻 校準(zhun)LED光源 Q-Box堅固外殼，用于儲(chu)存和運輸所有部(bu)件 |
DO電(dian)極(ji)，帶磁力攪拌器和LED光源 |
不同(tong)體積的(de)溶解氧反(fan)應杯電極 OX1LP-1 (1ml 有機玻璃) OX1LP-2.5 (2.5ml 玻(bo)璃 或有機玻(bo)璃) OX1LP-4 (4ml 有機玻璃) OX1LP-6 (6ml 玻璃) OX1LP-30 (30ml 有(you)機玻(bo)璃) OX1LP-50 (50ml有機玻璃或玻璃) |
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軟件界面及數據
客戶評價
“I have tested my new Qubit system with isolated oyster mitochondria, and was extremely pleased with the results. I can’t believe I was suffering for the whole year with my old chamber. I have tried three different systems based on Clarke-type electrodes including this one, and this is by far the most convenient for use with small sample volumes and gives a very clean, repeatable trace with the best signal to noise ratio I ever had with micro-chambers.”
From Dr. Inna Sokolova, Assistant Professor, Biology Department, University of North Carolina at Charlotte
“我用分離的牡蠣線粒(li)體(ti)測試了(le)Qubit系(xi)統，對結(jie)果(guo)非常滿(man)意(yi)。我真(zhen)不敢相(xiang)信忍受(shou)了整(zheng)整(zheng)一年舊(jiu)培(pei)養室(shi)。我嘗試了三種(zhong)不同類(lei)型的(de)克拉克電極，包括這一種(zhong)，這是迄今為止(zhi)測量(liang)小樣本很(hen)(hen)方便的(de)系(xi)統，提供了一種(zhong)非常干凈(jing)、可(ke)重復(fu)的(de)軌跡，且在微型培(pei)養室(shi)中信噪比很(hen)(hen)好。”
——北卡羅來(lai)納大學生(sheng)物系助理教授(shou) Dr. Inna Sokolova
發表文獻
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· Sussarellu, Rossana, et al. Rapid mitochondrial adjustments in response to short-term hypoxia and re-oxygenation in the Pacific oyster, Crassostrea gigas.” The Journal of Experimental Biology 216.9 1561-1569 (2013).
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· Bryce A. Mendelsohn, Bethany L. Kassebaum and Jonathan D. Gitlin. The zebrafish embryo as a dynamic model of anoxia tolerance. Developmental Dynamics Vol 237, Issue 7, p1780–1788 (2008).
· Bryce A. Mendelsohn and Jonathan D. Gitlin. Coordination of development and metabolism in the pre-midblastula transition zebrafish embryo. Developmental Dynamics Vol 237, Issue 7, p1789–1798(2008).
· Johnson EA, Rosenberg J, McCarty RE. Expression by Chlamydomonas reinhardtii of a chloroplast ATP synthease with polyhistidine-tagged beta subunits. Biochimica et Biophysica Acta 1767:374-380 (2007)
· Johnson E A. Altered expression of the chloroplasts ATP synthase through site-directed mutagenesis inChlamydamonas Reinhardtii. Photosynth Res vol 96:153-162 (2008)
· Charles W. Locuson, Peter M. Gannett and Timothy S. Tracy. Heteroactivator effects on the coupling and spin state equilibrium of CYP2C9. Archives of Biochemistry and Biophysics Vol 449, Issues 1-2, p115-129 (2006).
產地：加拿大
