簡介 Q-Box AQUA采(cai)用間歇(xie)流動呼(hu)吸法(fa),測量水生動物(wu)(脊椎動物(wu)和(he)無脊椎動物(wu)均(jun)可)的代謝速率(lv)。即將水生動物(wu)放置在一(yi)個呼(hu)吸室中,通(tong)過溶解氧的減少來衡(heng)量代謝速率(lv)。耗(hao)氧率(lv)(VO2)通(tong)過軟(ruan)件(jian)(jian)進行(xing)計算(suan),原始溶解氧數(shu)據經(jing)鹽度、溫(wen)度和(he)壓力校正,計算(suan)結果(guo)顯示并(bing)保存在軟(ruan)件(jian)(jian)中。 |
優點 l 間(jian)歇流動(dong)呼吸法無需將動(dong)物從(cong)試驗(yan)(yan)箱(xiang)中取出(chu),測量(liang)依次進(jin)行(xing),兩次測量(liang)之間(jian)用(yong)淡水沖洗試 驗(yan)(yan)箱(xiang),避免了傳統(tong) 密(mi)閉式測量(liang)方法導致的(de)呼吸時內(nei)產生(sheng)缺氧狀態。 l 數(shu)據(ju)準確,讀(du)數(shu)穩(wen)定,維護簡單(dan);· l 能長期地自動測量;· l 有不同(tong)大小呼吸室可以(yi)選擇,適宜(yi)于各種體積的水生動物測量 l 所有(you)部(bu)件統一安裝(zhuang)在堅固的運輸箱中,便于攜帶(dai)和(he)存(cun)放。 |
測量方式
在(zai)實驗(yan)過程中,水通過液流泵在(zai)動物室和帶光學溶解氧探針的(de)(de)流通容器中循環。由數字控制(zhi)單元(DCU)控制(zhi)三通閥(fa),軟(ruan)件控制(zhi)設定系統的(de)(de)循環或沖(chong)洗模式。
在循(xun)環模式下(xia)進行(xing)呼吸測量,在沖洗模式下(xia)用水(shui)浴中(zhong)的含氧水(shui)沖洗動物(wu)室。循(xun)環-沖洗-循(xun)環,重復運行(xing),直到實驗結束。沖洗和(he)循(xun)環階(jie)段(duan)的時間由用戶(hu)在軟件(jian)中(zhong)選擇(ze)。
用于小樣本的mini-AQUA
對于(yu)小(xiao)型(xing)(xing)魚(yu)類和昆蟲(chong)幼蟲(chong)(~1g)的水(shui)生呼吸(xi)測(ce)(ce)量,Qubit提供了mini-AQUA套裝。改良(liang)版的Q-Box Aqua帶(dai)有小(xiao)型(xing)(xing)水(shui)生呼吸(xi)室(9ml)和用于(yu)更小(xiao)系統體(ti)積的組件。還包括一(yi)個微型(xing)(xing)室(體(ti)積為(wei)1.23ml),用于(yu)測(ce)(ce)量小(xiao)于(yu)0.5g的幼蟲(chong)、卵和幼魚(yu)等非常小(xiao)的樣品(pin)的耗(hao)氧率VO2。在測(ce)(ce)量期(qi)間(jian)(jian),微型(xing)(xing)室采用間(jian)(jian)歇停止流(liu)動模(mo)式,并將溶(rong)解氧探針直接放置在該(gai)室中,從而提高系統的靈敏度和分辨(bian)率。
v 4個數(shu)據通道
v 可變的數據采集速率
v 圖表、表格和儀表顯示
v 分析(xi)功(gong)能套件:
(統計、曲(qu)線擬(ni)合(he)、積分等)
v 可(ke)輸入數據控制設備。
v 可生成多頁(ye)報告
v 數據(ju)回放功(gong)能
v 可輸(shu)入和編(bian)輯(ji)簡單公(gong)式
v 自動多種(zhong)數學函數
v 動(dong)態自動(dong)計算
應用領域
v 水生生物呼(hu)吸(xi)作用研究(jiu)(測試溫度、缺氧(yang)、養分、壓力(li)、體(ti)型對呼(hu)吸(xi)作用影(ying)響);
v 脊椎動物和無脊椎動物研(yan)究
v 實驗室或野外均可應用
基本組件
v 光學DO探頭(tou)和水浴
v 小型呼吸(xi)測量室(140ml)
v 大呼(hu)吸測(ce)量(liang)室(660ml)
v 水浴用(yong)溫鹽探(tan)頭(tou)
v 液體泵(1 LPM)和三(san)通閥
v 三通閥數(shu)字(zi)控(kong)制單元
v 4通道數據接(jie)口
v 數據采集軟(ruan)件
v 絕對(dui)壓力傳感器
v 堅固的防(fang)風雨外殼
v 可選電池組
技術參數
客戶評價
“We have been using the Q-Box AQUA respirometry package for a year and have been impressed with the quality and versatility of the system. Although designed for teaching, we have found it to be more than adequate for basic research applications on both fish and cephalopods. The mechanical systems are well designed and easily serviced and the software is intuitive and flexible enough for use in diverse applications. The technicians at Qubit are outstanding; they were able to modify the system to suit our particular needs and they have always been quick to respond when technical support was required. The fact that Qubit ships the system in a protective case was also a big plus for field work!”
form Dr. Tyson MacCormak, Biology Department, Mount Alison University, New Brunswick, Canada
“我們已經使用Q-Box AQUA呼吸測量軟件包一年了,它(ta)的質量和多功能(neng)性(xing)給我(wo)們(men)留下(xia)了深刻的印(yin)象。雖然是為(wei)教學而設(she)(she)計的,但我(wo)們(men)發現它(ta)對于(yu)魚(yu)類和頭足(zu)類的基礎研究應用來說已經(jing)足(zu)夠了。機械系統(tong)設(she)(she)計精良,維修方便,軟件直觀(guan)靈活(huo),適(shi)用于(yu)多種(zhong)應用。Qubit的技術人員非常出色(se);他們能(neng)夠(gou)修(xiu)改(gai)系統以滿足我們的特殊(shu)需要,且技術支持響應迅速。Qubit系(xi)統裝(zhuang)在一個(ge)保護箱里,非常有益于野外工作!
發表文獻
· Bessemer RA et al. (2015) Cardiorespiratory toxicity of environmentally relevant zinc oxide nanoparticles in the freshwater fish Catostomus commersonii. Nanotoxicology 9: 861-870
· Callaghan NI et al. (2016) Zinc oxide nanoparticles trigger cardiorespiratory stress and reduce aerobic scope in the white sucker, Catostomus commersonii. Nanoimpact 2:29-37
· Black MN et al. (2017) Environmentally relevant concentrations of amine-functionalized copper nanoparticles exhibit different mechanisms of bioactivity in Fundulus Heteroclitus in fresh and brackish water. Nanotoxicology 11:1070-1085
· Keogh C et al. (2017) The double edge to parasite escape: invasive host is less infected but more infectable. Ecology 98:2241-2247
· Rangel RE, Johnson DW (2018) Metabolic responses to temperature in a sedentary reef fish, the bluebanded goby (Lythrypnus dalli, Gilbert). Journal of Experimental Marine Biology and Ecology 501: 83-89
Lamarre SG et al. (2019) Interrelationship Between Contractility, Protein Synthesis and Metabolism in Mantle of Juvenile Cuttlefish (Sepia officinalis). Frontiers in Physiology V10, 1-14 (doi: 10.3389/fphys.2019.01051)
產地:加拿大