為更(geng)好地服務客戶,上海澤泉科技股份有(you)限公(gong)司(si)特邀德國(guo)WALZ資深光合熒光技術專家Gert Schansker博士,2019年(nian)11月將分別在上海、泰安、北京、沈陽等(deng)地舉(ju)辦系列巡回學術報(bao)告。
澤泉科技(ji)(ji)誠摯您參加(jia)本次活動(dong),交流光合熒光研究領域(yu)內的最(zui)新技(ji)(ji)術,期待您的光臨。
報告主題:葉綠素a熒光,PC、P700與(yu)(yu)Fd氧還(huan)狀態同步測(ce)量(liang)技術(shu)在植物抗(kang)逆,PSI與(yu)(yu)PSII間的互作,流經與(yu)(yu)環(huan)繞PSI的電子(zi)傳(chuan)遞(di)等研究中的應用(Application of simultaneous measurements of Chl a fluorescence and the redox states of PC, P700 and Fd to the study of the interaction between PS II and PS I, the electron flow through and around PS I as well as the screening for stress tolerance)
報告人:
Gert Schansker博士
報告時間與地點:
? 中科院上海植物生理生態研究所
2019年11月4日(ri)(周一) 下午13:30
地址:上(shang)(shang)海市徐匯區楓林路300號(hao)中科(ke)院上(shang)(shang)海植生(sheng)所1號(hao)樓113報告廳
? 山東農業大學
2019年11月6日(周三) 上午9:00
地址:山東(dong)省泰安(an)市(shi)岱宗(zong)大街(jie)61號山東(dong)農(nong)業(ye)大學國重(zhong)樓534報(bao)告廳(ting)
? 中科院北京植物研究所
2019年11月(yue)7日(周四) 下午15:10 – 16:40
地址:北京(jing)市(shi)香山南辛(xin)村(cun)20號(hao)中科院北京(jing)植物研究所景天樓303報告廳
? 沈陽農業大學
2019年(nian)11月11日(周一) 上午9:00
地(di)址(zhi):沈陽市東(dong)陵路(lu)120號沈陽農(nong)業大學園(yuan)藝(yi)學院326報告廳
報告摘要
作為(wei)PSI的(de)(de)電子(zi)供體(ti)(ti)(ti)和(he)電子(zi)受體(ti)(ti)(ti),PC(質(zhi)體(ti)(ti)(ti)藍素)和(he)Fd(鐵氧(yang)還(huan)(huan)蛋白)對PSI的(de)(de)氧(yang)化(hua)還(huan)(huan)原起著至關重(zhong)要(yao)的(de)(de)調控作用。但一(yi)直缺(que)乏科學便捷的(de)(de)手段(duan)對其運(yun)轉狀態(tai)進(jin)行檢測(ce)。集(ji)成以DUAL-PAM-100為(wei)標志的(de)(de)第二(er)代(dai)PAM的(de)(de)基本功能,采(cai)用先進(jin)的(de)(de)解(jie)(jie)卷積技(ji)術(shu)(一(yi)種根據來源不同(tong)對信號進(jin)行分離的(de)(de)技(ji)術(shu)),WALZ公司推出了可以測(ce)量(liang)PC和(he)Fd氧(yang)化(hua)還(huan)(huan)原狀態(tai)的(de)(de)新一(yi)代(dai)PAM熒光(guang)(guang)儀(yi)(yi)—DUAL-KLAS-NIR四(si)通道(dao)動態(tai)LED陣列(lie)近紅外光(guang)(guang)譜(pu)儀(yi)(yi)。新設備能夠測(ce)量(liang)4組不同(tong)波段(duan)(780-820nm,820-870nm,840-965nm,870-965nm)的(de)(de)信號,實現對P700(PSI反(fan)應中心)、PC和(he)Fd的(de)(de)氧(yang)化(hua)還(huan)(huan)原狀態(tai)分別測(ce)量(liang)。另外,它(ta)還(huan)(huan)可以測(ce)量(liang)由540nm和(he)460nm光(guang)(guang)化(hua)光(guang)(guang)激發的(de)(de)葉(xie)(xie)綠素熒光(guang)(guang),了解(jie)(jie)葉(xie)(xie)片(pian)深(shen)層熒光(guang)(guang)信息。
利用DUAL-KLAS-NIR四通道動態(tai)(tai)LED陣列近紅外光(guang)(guang)(guang)譜儀,可(ke)(ke)以準同步地(di)測量各種(zhong)不同的(de)(de)信(xin)號,不僅在馳(chi)豫動力(li)下(xia)(xia),還可(ke)(ke)持(chi)續地(di)在自然穩態(tai)(tai)下(xia)(xia)同時獲取各組分的(de)(de)信(xin)息。可(ke)(ke)獲取Fd和(he)PC庫(ku)相對(dui)于P700的(de)(de)大小及不同條件下(xia)(xia)各組分氧化還原的(de)(de)比例(li),了(le)解依賴類囊體(ti)腔內質子(zi)釋放和(he)ATP合酶的(de)(de)消耗之間平衡的(de)(de)光(guang)(guang)(guang)合控(kong)制過程等,在植物抗逆(ni)生(sheng)理(li)(li),光(guang)(guang)(guang)合突變體(ti)鑒定、PSI與(yu)PSII間的(de)(de)互(hu)作,流經與(yu)環繞(rao)PSI的(de)(de)電(dian)子(zi)傳(chuan)遞等光(guang)(guang)(guang)合機理(li)(li)與(yu)生(sheng)理(li)(li)生(sheng)態(tai)(tai)研(yan)究中(zhong)均(jun)有廣泛的(de)(de)應(ying)用。
參會聯系方式
鄭寶剛(gang):轉8011,Email:welkin.;
李俊艷:/76/77轉(zhuan)821,Email:tracy.;
識別下方二維碼,填寫信息免費參會。
專家簡介
Gert Schansker博士(shi)畢業于(yu)荷蘭瓦(wa)赫寧(ning)根(gen)大學(xue)(xue),獲(huo)得植(zhi)物生理(li)學(xue)(xue)和(he)生物物理(li)學(xue)(xue)博士(shi)學(xue)(xue)位(wei)。主(zhu)要(yao)研(yan)(yan)究(jiu)方(fang)向(xiang)為(wei)光(guang)(guang)(guang)(guang)合機(ji)(ji)構(gou)的(de)(de)(de)(de)(de)(de)(de)(de)光(guang)(guang)(guang)(guang)脅(xie)(xie)迫(po)反應(ying),提出光(guang)(guang)(guang)(guang)系(xi)統(tong)II受(shou)體(ti)側碳酸氫鹽(yan)的(de)(de)(de)(de)(de)(de)(de)(de)不可(ke)逆損(sun)失(shi)是(shi)(shi)光(guang)(guang)(guang)(guang)系(xi)統(tong)II活性(xing)降低(di)的(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)要(yao)發生機(ji)(ji)制。他在研(yan)(yan)究(jiu)中(zhong)應(ying)用(yong)的(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)要(yao)非侵入性(xing)技(ji)術(shu)之(zhi)(zhi)一是(shi)(shi)葉(xie)(xie)綠(lv)素(su)a熒光(guang)(guang)(guang)(guang)與光(guang)(guang)(guang)(guang)聲信號(hao)(hao)的(de)(de)(de)(de)(de)(de)(de)(de)同步測量技(ji)術(shu)。之(zhi)(zhi)后(hou)在歐盟的(de)(de)(de)(de)(de)(de)(de)(de)資助(zhu)下,前(qian)往希臘(la)雅典Demokritos研(yan)(yan)究(jiu)所從事(shi)博士(shi)后(hou)研(yan)(yan)究(jiu),使用(yong)EPR技(ji)術(shu)研(yan)(yan)究(jiu)一氧(yang)化氮(NO)與光(guang)(guang)(guang)(guang)系(xi)統(tong)II錳簇S態的(de)(de)(de)(de)(de)(de)(de)(de)相(xiang)互作(zuo)用(yong)。他利(li)用(yong)一系(xi)列(lie)單周(zhou)轉飽和(he)閃光(guang)(guang)(guang)(guang)及葉(xie)(xie)綠(lv)素(su)熒光(guang)(guang)(guang)(guang)Fo信號(hao)(hao)與S態相(xiang)關(guan)的(de)(de)(de)(de)(de)(de)(de)(de)周(zhou)期-4振幅研(yan)(yan)究(jiu)了(le)S態與S態衰變(bian)對NO的(de)(de)(de)(de)(de)(de)(de)(de)響應(ying),闡明了(le)實(shi)驗中(zhong)觀測到(dao)的(de)(de)(de)(de)(de)(de)(de)(de)NO誘導的(de)(de)(de)(de)(de)(de)(de)(de)多線態EPR信號(hao)(hao)可(ke)能就是(shi)(shi)S-2態的(de)(de)(de)(de)(de)(de)(de)(de)表(biao)征。后(hou)來在瑞士(shi)日(ri)內瓦(wa)Reto Strasser博士(shi)的(de)(de)(de)(de)(de)(de)(de)(de)實(shi)驗室工(gong)作(zuo)期間(jian),研(yan)(yan)究(jiu)了(le)光(guang)(guang)(guang)(guang)暗轉換過(guo)程(cheng)中(zhong)820 nm吸收信號(hao)(hao)與葉(xie)(xie)綠(lv)素(su)a熒光(guang)(guang)(guang)(guang)動力學(xue)(xue)之(zhi)(zhi)間(jian)的(de)(de)(de)(de)(de)(de)(de)(de)關(guan)系(xi),系(xi)統(tong)研(yan)(yan)究(jiu)了(le)多種(zhong)植(zhi)物在各種(zhong)脅(xie)(xie)迫(po)條件(jian)下的(de)(de)(de)(de)(de)(de)(de)(de)快速葉(xie)(xie)綠(lv)素(su)熒光(guang)(guang)(guang)(guang)誘導動力學(xue)(xue)曲線(O-I1-I2-P或O-J-I-P瞬變(bian)),為(wei)此類測量提供了(le)幾(ji)乎(hu)完整的(de)(de)(de)(de)(de)(de)(de)(de)描(miao)述(shu)。在匈牙利(li)結束了(le)光(guang)(guang)(guang)(guang)適(shi)應(ying)和(he)一種(zhong)蝦青素(su)過(guo)量導致煙草突變(bian)的(de)(de)(de)(de)(de)(de)(de)(de)研(yan)(yan)究(jiu)之(zhi)(zhi)后(hou),自2018年(nian)開(kai)始,Gert Schansker博士(shi)作(zuo)為(wei)德國WALZ公司的(de)(de)(de)(de)(de)(de)(de)(de)應(ying)用(yong)科學(xue)(xue)家(jia),負責Multi-Color-PAM和(he)Dual-KLAS-NIR相(xiang)關(guan)理(li)論和(he)應(ying)用(yong)的(de)(de)(de)(de)(de)(de)(de)(de)研(yan)(yan)究(jiu)工(gong)作(zuo)。
報告人近年發表的文章
1. Schansker G, Tóth S Z, Holzwarth A R, et al. Chlorophyll a fluorescence: beyond the limits of the Q A model[J]. Photosynthesis research, 2014, 120(1-2): 43-58.
2. Schansker G, Tóth S Z, Kovács L, et al. Evidence for a fluorescence yield change driven by a light-induced conformational change within photosystem II during the fast chlorophyll a fluorescence rise[J]. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 2011, 1807(9): 1032-1043.
3. Schansker G, Yuan Y, Strasser R J. Chl a fluorescence and 820 nm transmission changes occurring during a dark-to-light transition in pine needles and pea leaves: a comparison[M]//Photosynthesis. Energy from the Sun. Springer, Dordrecht, 2008: 945-949.
4. Schansker G, Tóth S Z, Strasser R J. Dark recovery of the Chl a fluorescence transient (OJIP) after light adaptation: the qT-component of non-photochemical quenching is related to an activated photosystem I acceptor side[J]. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 2006, 1757(7): 787-797.
5. Schansker G, Tóth S Z, Strasser R J. Methylviologen and dibromothymoquinone treatments of pea leaves reveal the role of photosystem I in the Chl a fluorescence rise OJIP[J]. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 2005, 1706(3): 250-261.
6. Schansker G, Strasser R J. Quantification of non-Q B-reducing centers in leaves using a far-red pre-illumination[J]. Photosynthesis research, 2005, 84(1-3): 145-151.
7. Schansker G, Srivastava A, Strasser R J. Characterization of the 820-nm transmission signal paralleling the chlorophyll a fluorescence rise (OJIP) in pea leaves[J]. Functional Plant Biology, 2003, 30(7): 785-796.
8. Schansker G, Goussias C, Petrouleas V, et al. Reduction of the Mn cluster of the water-oxidizing enzyme by nitric oxide: formation of an S-2 state[J]. Biochemistry, 2002, 41(9): 3057-3064.
9. Schansker G, Van Rensen J J S. Performance of active photosystem II centers in photoinhibited pea leaves[J]. Photosynthesis Research, 1999, 62(2): 175-184.