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草業科學專用的葉綠素熒光成像系統Weed-PAM
日期:2018-05-09 14:53:00

草業科學-葉(xie)綠素熒光成像系(xi)統(tong)Weed-PAM

基于(yu)功能強大的IMAG-PAM,改進升(sheng)級后(hou)適用于野外(wai)雜(za)草,草坪等光(guang)合作用研究

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主要(yao)功能(neng)

l 成像功能:對FtFoFmFv/FmFFm’Y(II)Y(NO)Y(NPQ)NPQqNqPqLETRAbs.NIRRed等至少17種參數進行成像分析。測定調節性能量耗散Y(NPQ),反映植(zhi)物光保護能(neng)(neng)力(li),測定(ding)非調節性能(neng)(neng)量耗散(san)Y(NO),反映植(zhi)物(wu)光損傷程度。

l 程序測量功能:可程序測(ce)量熒(ying)光(guang)(guang)誘導(dao)曲(qu)(qu)線(xian)、快速(su)光(guang)(guang)曲(qu)(qu)線(xian)和暗弛豫,也(ye)可手(shou)動測(ce)量;在測(ce)量過程中能自(zi)動分(fen)析所(suo)有熒(ying)光(guang)(guang)參數的變化趨勢

l AOI功能:可在測(ce)量(liang)前或測(ce)量(liang)后任意選(xuan)擇(ze)感興趣的區(qu)域(AOI),程序將自動(dong)對選擇的AOI的數(shu)據進(jin)行變化趨勢(shi)分(fen)析,并(bing)在報(bao)告文件中(zhong)顯(xian)示相關(guan)AOI的(de)數(shu)據。所有報告文(wen)件中顯(xian)示的(de)數(shu)據都可(ke)導出(chu)到EXCEL文件中。

l 成像異質性分(fen)析功(gong)能:對任(ren)意參數(shu)任(ren)意時間的成(cheng)像(xiang),可在圖像(xiang)上任(ren)意選取兩(liang)點(dian),軟件自(zi)動(dong)對兩(liang)點(dian)間的數(shu)據進(jin)行橫向異(yi)質性分析,并可導(dao)出到EXCEL文件中。

l 成像數據范圍分析功能:對任(ren)意參(can)數任(ren)意時間(jian)的成(cheng)像,可分析任(ren)意兩個熒光數值之間(jian)有(you)多少個像素(su)點,多少面積(cm2)。

l 突變株篩選功能:可跟據(ju)成像結果快速(su)篩(shai)選光合、產氫/油、抗逆(抗鹽、抗旱、抗病等)等突變株

l 吸(xi)光系(xi)數(shu)測量功能:快速測(ce)量(liang)葉片的吸光系數。吸光系數測(ce)量(liang)光源: 16個(ge)紅光(guang)(650 nm)和16個近紅外(wai)(780 nmLED,用于測量植物葉片或藻類樣品PAR吸光系數。

成像(xiang)參數

Fo, Fm, F, Ft, Fm', Fv/Fm, Y(II), qL, qP, qN, NPQ, Y(NPQ), Y(NO), ETR, Abs, NIR和Red等

技術參數

Maxi探頭(藍光版)藍色LED,450 nm


Maxi探頭(tou)(紅光版)紅色LED650 nm


Mini探頭(藍光版(ban)藍色LED460 nm

 

Mini探頭(紅光版紅色LED620 nm


應用領域

光合作用研究:可以在相同的條件下同時對大量樣品進行成像

l 植物病理學:病斑(ban)部位(wei)(包括(kuo)肉眼不可(ke)見時)成像(xiang)以(yi)及病斑(ban)擴散(san)的時空動力學(xue)

l 植物脅迫生 理學(xue):肉眼不可(ke)見(jian)脅迫損(sun)傷的早期檢測

l 遺(yi)傳(chuan)育種(zhong):出苗后大規模(mo)快速(su)篩選高光合(he)/抗旱/抗熱/抗凍/抗病(bing)等植株

l 突變株(zhu)篩選:快速篩選模式植物的(de)光(guang)合突(tu)(tu)變(bian)株、抗逆突(tu)(tu)變(bian)株、產(chan)氫(qing)微藻(zao)突(tu)(tu)變(bian)株等

l 分子生物學:宏觀水(shui)平上檢測樣品的綠(lv)色熒光蛋白(GFP)熒(ying)光

l 其它(ta)多種擴展研究(jiu)



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參考文獻

數(shu)據來(lai)源:光合作用文獻(xian) Endnote 數(shu)據庫,更新至 2021 年 1 月,文獻(xian)數(shu)量(liang)超過 10000 篇(pian)

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