功(gong)能強(qiang)大、操作簡便、文獻眾(zhong)多的葉綠素熒光(guang)成(cheng)像系(xi)統
突變(bian)株(zhu)快速篩選的強大工(gong)具
? 標準藍光版(ban)，450 nm，測葉片和真核藻類 ? 紅(hong)光版，620 nm，測(ce)藍(lan)藻和真核藻類 |
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主要功能
? 成(cheng)像功能：對(dui)Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等參數(shu)進行(xing)成(cheng)像分析。測(ce)定調節性(xing)(xing)能量耗散Y(NPQ)，反(fan)映(ying)植物光保護能力，測(ce)定非調節性(xing)(xing)能量耗散Y(NO)，反(fan)映(ying)植物光損傷(shang)程(cheng)度。
? 程(cheng)序測(ce)量功能：可(ke)程(cheng)序測(ce)量熒光誘導曲線(xian)、快(kuai)速光曲線(xian)和暗(an)弛(chi)豫，也可(ke)手動(dong)測(ce)量；在測(ce)量過(guo)程(cheng)中能自動(dong)分(fen)析所(suo)有(you)熒光參數的變化趨勢
? AOI功能(neng)：可在測(ce)量前(qian)或測(ce)量后任意選擇(ze)感興趣的(de)(de)(de)區域（AOI），程序(xu)將自動對選擇(ze)的(de)(de)(de)AOI的(de)(de)(de)數據(ju)(ju)進(jin)行變化趨(qu)勢分(fen)析，并(bing)在報告文(wen)件(jian)(jian)中顯(xian)示相關AOI的(de)(de)(de)數據(ju)(ju)。所有報告文(wen)件(jian)(jian)中顯(xian)示的(de)(de)(de)數據(ju)(ju)都可導(dao)出(chu)到EXCEL文(wen)件(jian)(jian)中。
? 成(cheng)像異質性分(fen)析功(gong)能：對(dui)任(ren)意參數任(ren)意時間的成(cheng)像，可在圖(tu)像上任(ren)意選取兩點(dian)，軟件(jian)自動(dong)對(dui)兩點(dian)間的數據(ju)進行(xing)橫向異質性分(fen)析，并可導出到EXCEL文(wen)件(jian)中(zhong)。
? 成像(xiang)數據范圍(wei)分析(xi)功能：對任意(yi)(yi)參數任意(yi)(yi)時間的成像(xiang)，可分析(xi)任意(yi)(yi)兩(liang)個熒光數值之(zhi)間有多少(shao)個像(xiang)素(su)點，多少(shao)面積（cm2）。
? 突變株篩選功能：可跟據成像結果快速篩選光合、產氫/油、抗逆（抗鹽、抗旱、抗病等）等突變株。
? 微(wei)藻毒理研究功能：可同時測量(liang)96個微(wei)藻樣品（對照和處(chu)(chu)理組(zu)(zu)）的光(guang)合(he)(he)活性，軟件自動給出處(chu)(chu)理組(zu)(zu)樣品相(xiang)對于對照組(zu)(zu)的光(guang)合(he)(he)抑制百(bai)分(fen)比(bi)。
? 吸光(guang)(guang)(guang)系(xi)(xi)數測(ce)量功(gong)能：快速測(ce)量葉片的吸光(guang)(guang)(guang)系(xi)(xi)數。吸光(guang)(guang)(guang)系(xi)(xi)數測(ce)量光(guang)(guang)(guang)源： 16個紅(hong)光(guang)(guang)(guang)（650 nm）和16個近紅(hong)外（780 nm）LED，用于測(ce)量植物葉片或藻類樣品PAR吸光(guang)(guang)(guang)系(xi)(xi)數。
? 監(jian)(jian)(jian)測功能：可(ke)實時(shi)監(jian)(jian)(jian)控箱內(nei)環境狀態、機器狀態監(jian)(jian)(jian)控區(qu)顯示(shi)目前程(cheng)序及(ji)環境狀態
? 環境(jing)仿真(zhen)功能(neng)：可程(cheng)序(xu)(xu)化多(duo)組程(cheng)序(xu)(xu)功能(neng) (可多(duo)至6組)，可個(ge)別(bie)設(she)定執行天數，可設(she)備單日環境(jing)參(can)數（間隔可至分鐘），程(cheng)序(xu)(xu)化環境(jing)參(can)數
? 項目管理功能：預(yu)約存多(duo)次測量的(de)植物影(ying)像及參數(shu)，延(yan)時(shi)（縮時(shi)）攝影(ying)功能
? 數(shu)據分析功能：可(ke)導(dao)(dao)出(chu)照片文件，可(ke)導(dao)(dao)出(chu)葉綠(lv)素熒光(guang)參(can)數(shu)Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等(deng)
系統設計
? 可(ke)單(dan)獨(du)作為葉綠(lv)素(su)熒光(guang)儀使用，方便帶到野外(wai)田(tian)間測(ce)量(liang)(liang)和(he)(he)(he)成(cheng)像；也可(ke)配置箱(xiang)(xiang)體式結構，容量(liang)(liang)300L，能同時滿(man)足(zu)植(zhi)物(wu)培養和(he)(he)(he)葉綠(lv)素(su)熒光(guang)成(cheng)像的功能。箱(xiang)(xiang)體內(nei)(nei)設有栽(zai)培區(qu)，可(ke)分2層(ceng)(ceng)、3層(ceng)(ceng)、4層(ceng)(ceng)等(deng)，調節高度進行培養和(he)(he)(he)測(ce)量(liang)(liang)。箱(xiang)(xiang)體內(nei)(nei)有特殊消(xiao)光(guang)圖層(ceng)(ceng)，避免反(fan)光(guang)造(zao)成(cheng)的測(ce)量(liang)(liang)干擾。
? 系統配置觸摸(mo)式(shi)高清顯示(shi)屏（24寸(cun)），方便操作，支(zhi)持1670萬色彩和(he)FHD高清畫質。
? 系(xi)統處理器：I7處理器，16G緩存，512固態硬盤SSD，滿足快速數(shu)據處理和存儲要求。
? 供(gong)電(dian)：室內使(shi)用220V，50Hz交流電(dian)，熒光(guang)儀單獨使(shi)用時，內置可充電(dian)鋰電(dian)池供(gong)電(dian)，滿(man)足4小(xiao)時的野外(wai)測量
箱體環境控制（光照、溫度等）
? 溫(wen)度環(huan)控條件(jian)：15-40°C ±5°C（關燈）
? 光照(zhao)系(xi)統：采用(yong)四合(he)一LED光盤，白(bai)光5000k ±500k、紅光660nm±10nm、藍450nm±10nm、紅外(wai)光730nm±10nm，光盤面積29×40cm，四種光源(yuan)可(ke)獨立自(zi)主控制(zhi)，光質強度(du)可(ke)達PPFD ≧700μmols-1m-2@15cm，光照(zhao)時間控制(zhi)可(ke)程序化設(she)(she)定，設(she)(she)定時間可(ke)至1min，可(ke)仿真日出至日落光照(zhao)變化。
? 控制(zhi)系統(tong)：PID微電腦控制(zhi)，可設定上(shang)晝夜循環程序
? 可(ke)選配(pei)二氧化碳和濕(shi)度(du)控制單元(yuan)
成像技術參數
Maxi探頭
? 熒(ying)光(guang)測(ce)(ce)量光(guang)源(yuan)： 44個(ge)藍色LED，450 nm，測(ce)(ce)量光(guang)強度(du)0.5 μmol m-2 s-1PAR，最(zui)大光(guang)化光(guang)強度(du)1900 μmol m-2 s-1PAR，飽和脈(mo)沖強度(du)4000 μmol m-2 s-1PAR
? 吸(xi)光(guang)系數測量光(guang)源(yuan)：16個(ge)(ge)紅(hong)光(guang)（660 nm）和(he)16個(ge)(ge)近(jin)紅(hong)外（780 nm）LED，用于測量樣品(pin)PAR吸(xi)光(guang)系數。
? 成(cheng)像面(mian)積：工作距(ju)離(li)18.5cm，成(cheng)像面(mian)積 10×13 cm；工作距(ju)離(li)22.5cm，成(cheng)像面(mian)積11×15cm。
? 光(guang)強異(yi)質(zhi)性(xing)：測量區域光(guang)強異(yi)質(zhi)性(xing)小于±7％。
? 測量參數(shu)：Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等(deng)。
應用領域
? 光合作用研(yan)究：可以(yi)在(zai)完(wan)全相同的條件下同時對大量樣(yang)品(pin)進行成(cheng)像
? 植物病理學(xue)：病斑部位(wei)（包括肉眼不可(ke)見時）成像(xiang)以及(ji)病斑擴散的(de)時空動力學(xue)
? 植物脅(xie)(xie)迫生 理(li)學：肉(rou)眼不可見脅(xie)(xie)迫損傷的早(zao)期檢(jian)測
? 遺傳育種：出苗(miao)后大規模快速篩選高光合/抗(kang)(kang)旱/抗(kang)(kang)熱/抗(kang)(kang)凍/抗(kang)(kang)病等植株
? 突(tu)變(bian)(bian)株篩選：快(kuai)速篩選模式植物(wu)的光合突(tu)變(bian)(bian)株、抗逆突(tu)變(bian)(bian)株、產(chan)氫微藻突(tu)變(bian)(bian)株等(deng)
? 微(wei)藻(zao)毒理學：不同毒物濃度多個(ge)重復的(de)樣品一次測(ce)完，軟件自(zi)動(dong)計算抑制比率(lv)
? 分子生物學：宏觀水平上檢測樣品的綠色(se)熒光蛋白（GFP）熒光
? 其它多種擴展研(yan)究
成像參數
Fo, Fm, F, Ft, Fm', Fv/Fm, Y(II), qL, qP, qN, NPQ, Y(NPQ), Y(NO), ETR, Abs, NIR和Red等
突變株的快速篩選
MAXI-IMAGING-PAM特別適合(he)對幼(you)苗、愈傷(shang)組織、微藻等(deng)進(jin)行突(tu)(tu)變(bian)(bian)株(zhu)的快速篩選，適合(he)于與(yu)光合(he)突(tu)(tu)變(bian)(bian)株(zhu)、抗逆(ni)（抗旱(han)、抗鹽、抗病等(deng)）突(tu)(tu)變(bian)(bian)株(zhu)、產油/氫突(tu)(tu)變(bian)(bian)株(zhu)等(deng)的快速篩選。
國(guo)外利用MAXI-IMAGING-PAM篩選突變株的典型(xing)(xing)客戶如(ru)(ru)拜耳、BASF、孟山都(dou)、先正達(da)等(deng)大(da)(da)型(xing)(xing)跨國(guo)農業巨頭，以及各(ge)大(da)(da)農業育種、植(zhi)物分子(zi)生物學(xue)等(deng)科研單位，例如(ru)(ru)澳大(da)(da)利亞植(zhi)物功能基因組中心（阿德(de)雷德(de)大(da)(da)學(xue)）、德(de)國(guo)尤(you)里(li)希表(biao)型(xing)(xing)植(zhi)物表(biao)型(xing)(xing)研究中心（Julich Plant Phenotyping Centre）等(deng)等(deng)。
國(guo)內約一(yi)半的MAXI-IMAGING-PAM客戶在進行突變株快(kuai)速篩選(xuan)工(gong)作，主(zhu)要分(fen)布于中國(guo)科學(xue)院、中國(guo)農(nong)科院和各(ge)大(da)高校。
突(tu)變株篩選(xuan)實例一：國內某客戶篩選(xuan)的擬(ni)南(nan)芥突(tu)變株
突(tu)變(bian)株(zhu)篩選(xuan)實例二：產(chan)油突(tu)變(bian)株(zhu)的(de)篩選(xuan)。Ajjawi et al, 2010, Plant Physiol., 152: 529-540.
突(tu)變株(zhu)篩選(xuan)(xuan)實例三：光合突(tu)變株(zhu)的篩選(xuan)(xuan)。Armbruster et al., 2010, Plant Cell, 22: 3439-3460.
調制葉綠素熒光成像實例
葉片成像異質性 1）葡萄葉片
2）荷花葉片
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水果的成像 1）草莓的成像
2）獼猴桃的成像
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突變株篩選
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植物病理研究
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熒光成像與CO2氣體交換的同步測量
（GFS-3000/IM-MAXI）：MAXI-探頭與(yu)GFS-3000聯用，在10 cm x 13 cm的(de)面積上同步測量氣體交換與(yu)熒光成像(xiang)。
產地：德國WALZ
代表文獻
數據來源：光合(he)作(zuo)用文獻Endnote數據庫
原始數據來源：Google Scholar
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