主要功能
本系統是全球檢出限和靈(ling)敏度(du)很高的乙烯監測(ce)系統，主(zhu)要用(yong)于(yu)植(zhi)物(wu)研(yan)究相(xiang)(xiang)關(guan)的乙烯氣體監測(ce)，如種子發芽、植(zhi)物(wu)生長發育(yu)、開(kai)花生理、植(zhi)物(wu)器(qi)官衰(shuai)老、基因表達、植(zhi)物(wu)病原體相(xiang)(xiang)互(hu)作(zuo)用(yong)、植(zhi)物(wu)激素間相(xiang)(xiang)互(hu)作(zuo)用(yong)、蔬果收貨后保藏、植(zhi)物(wu)抗逆性(xing)研(yan)究（干(gan)旱、高溫、重金屬）等。
其中乙烯氣體檢測儀 ETD-300 采用先進的激光技術（光聲學原理），即樣品乙烯在光聲腔吸收激光后釋放熱使光聲腔內部產生壓力，隨激光頻率增減形成能被微型麥克風檢測到的壓力差，而乙烯濃度越高壓力差越大，從而據聲波強度差可實時快速測量乙烯氣體（C2H4）絕對濃度；閥門控制箱 VC-6 完全自動化和電腦控制，接一個即可以使單個氣體檢測儀實現6個樣品的自動切換測量，單個乙烯氣體檢測儀可以接一個或多個閥門控制箱；烴分解器 CAT-1 則利用鉑金顆粒催化烴氧化分解為水蒸氣和 CO2，為系統提供無烴干擾的樣品空氣。
測量參(can)數
測量(liang)參數：乙烯濃(nong)度（ppbv）、氣體流速（l/h）、背(bei)景值、模(mo)擬輸入（V）
計算參數：乙烯產(chan)量（nl/h）
連續流(liu)動測(ce)定（左）和積累測(ce)定（右(you)）的乙烯監測(ce)數據(ju)圖
應用領(ling)域
用于環境(jing)、醫學(xue)、農業、工業、生(sheng)態、生(sheng)物(wu)等(deng)監測領域。特(te)別適(shi)合植物(wu)生(sheng)理、發育研究的超靈敏乙烯測量(liang)。
主要(yao)技術參數(shu)
參數(shu) | 乙烯氣體檢測儀 ETD-300 | 閥門控(kong)制(zhi)箱 VC-6 | 烴(jing)分解器(qi) CAT-1 | |
測量(liang)范圍(wei) | 0-2 ppm / 0-100 ppm（可調） | / | / | |
檢出限(xian) | 0.3 ppbv | / | / | |
噪(zao)音(2σ) | 0.3 ppbv | / | / | |
精度 | <1% 或 0.3 ppbv | 0.2% FS | / | |
穩定性 | <1% 超過 24 小時 | / | / | |
零(ling)點漂移 | +/-1 ppbv | / | / | |
測量時間(jian) | 7-9 s | / | / | |
響(xiang)應時間 | 30 s （當(dang)流(liu)量(liang)為1 l/h時） | 300 ms | / | |
流量 | 0.25-5 l/h | 0.25-5 l/h | 0-30 l/h | |
校準 | 使用標準混合氣，每年一次 | / | / | |
通道數量 | / | 6（可增(zeng)至 12, 18 等） | / | |
測量(liang)模(mo)式(shi) | / | 連(lian)續測量(liang)，積累測量(liang) | / | |
氣體供應壓(ya)力(li) | / | 0.5-5 Bar | / | |
過壓閥 | / | 在 5 Bar 時(shi)打開 | / | |
濾膜類型 | / | 去除粒徑(jing) >7μm 的微(wei)粒 | / | |
最大稀(xi)釋濃度 | / | / | 100 ppm | |
輸出濃(nong)度 | / | / | < 100 pptv | |
壓力(li) | / | / | 0-6 atm | |
活性催(cui)化劑 | / | / | Pt/SiO2 | |
催化溫度 | / | / | 150–250 ℃ | |
預熱時間 | 30 min | / | < 10 min | |
尺寸 | 42x45x14 cm (48.3cm 3U 機(ji)架) | 30x45x10 cm (48.3cm 2U機架) | 33x24x14 cm （48.3 cm 3U 半機架） | |
工作溫度/濕度 | 10-28 ℃ / 0-95 % RH | 5-40 ℃ / 0-95 % RH | 5-40 ℃ / 0-95 % RH | |
電源要求(qiu) | 90-264 VAC，47-63 Hz | 90-264 VAC，47-63 Hz | 90-264 VAC，47-63 Hz | |
功耗 | <150 W | <20 W | 85 W | |
進氣接口 | 接外徑 1/8'' 軟管的快速接頭 | 接外徑 1/8'' 軟管(guan)的(de)快速(su)接頭 | 接外徑 1/8'' 軟管的快速接頭 | |
模擬(ni)輸入 | 0-5 V | / | / | |
數(shu)據(ju)輸(shu)出 | USB，CSV 格(ge)式 | USB，CSV 格式 | / | |
顯(xian)示 | 觸摸屏 | LED 指示(shi)燈(deng) | / |
選購指南：
6通道監測系統組成如下：
乙烯氣體檢測儀ETD-300 + 閥門控制箱VC-6 + 烴分解器CAT-1
注：系統中 3 個儀(yi)器都可以單獨(du)使用
可酌情(qing)選擇單(dan)通道系(xi)統：乙烯(xi)氣體檢(jian)測儀 ETD-300+ 烴分解器 CAT-1。
產地：荷蘭Sensor Sense 
應用舉例(li)
1.1 乙烯(xi)測定在(zai)高溫脅迫研究中的應(ying)用(yong)舉例(li)
實驗內容簡(jian)介：以生(sheng)(sheng)長 3 周的擬南芥野生(sheng)(sheng)型(xing) Col-0，突變(bian)體(ti) NahG 和 opr3 植株為材料，研究(jiu)了其高溫(wen)脅(xie)迫(po)下(xia)的乙(yi)烯(xi)釋放(fang)(fang)。其中，野生(sheng)(sheng)型(xing) Col-0 高溫(wen)脅(xie)迫(po)（38℃）下(xia)，電導(dao)率（電解質滲透率）、水楊酸(suan)和茉(mo)(mo)莉(li)酸(suan)含(han)量和乙(yi)烯(xi)釋放(fang)(fang)增(zeng)加(jia)；突變(bian)體(ti) NahG 和 opr3 高溫(wen)脅(xie)迫(po)（38℃）下(xia)電導(dao)率、茉(mo)(mo)莉(li)酸(suan)和乙(yi)烯(xi)釋放(fang)(fang)也增(zeng)加(jia)，但都低于(yu)(yu)野生(sheng)(sheng)型(xing) Col-0，而高溫(wen)脅(xie)迫(po)后恢復階段(duan)（水中 22℃）電導(dao)率明顯(xian)高于(yu)(yu) Col-0。研究(jiu)結(jie)果表明：高溫(wen)脅(xie)迫(po)下(xia)，乙(yi)烯(xi)迅速產(chan)生(sheng)(sheng)，其生(sheng)(sheng)產(chan)受到茉(mo)(mo)莉(li)酸(suan)和水楊酸(suan)的調(diao)控。總的來說，茉(mo)(mo)莉(li)酸(suan)與水楊酸(suan)協同(tong)調(diao)節植物對高溫(wen)脅(xie)迫(po)的耐受，而乙(yi)烯(xi)主要加(jia)快細胞死亡；突變(bian)體(ti) NahG 和 opr3 比野生(sheng)(sheng)型(xing) Col-0 的耐熱性差(cha)，細胞死亡多。
圖1 高(gao)溫(wen)處理(li)下擬南芥植(zhi)株(zhu)的水(shui)楊酸（a）、電(dian)導率（b、c）和乙烯釋放（d、e）
WT：擬南芥野生型；突變株opr3 ；突變株NahG以及培養基agar
Clarke, S.M., et al., Jasmonates act with salicyli c acid to confer basal thermotolerance in Arabidopsis thaliana. New Phytologist, 2009. 182(1): p. 175-187.
1.2 乙烯測(ce)定在營養缺(que)乏(fa)（Mg）脅迫(po)研(yan)究中的應用舉例
實驗內容簡介：以生長5周的(de)(de)(de)水培(pei)擬南芥(jie) Col-0 植(zhi)(zhi)株為(wei)材料，研(yan)究(jiu)了(le)其缺(que)鎂(mei)脅(xie)迫下的(de)(de)(de)乙(yi)烯釋放(fang)。缺(que)鎂(mei)處(chu)理后乙(yi)烯生物合成酶(mei)(mei)基因（例(li)如 At5g43450、At1g06620 和At2g25450）的(de)(de)(de)表(biao)達水平明顯上升，樣品乙(yi)烯釋放(fang)是(shi)對(dui)照組(zu)的(de)(de)(de)兩(liang)倍多(duo)，葉片中(zhong)抗壞血酸 ASC 和谷胱甘肽 GSH 的(de)(de)(de)氧(yang)化(hua)態(tai)比例(li)增加。研(yan)究(jiu)結果表(biao)明：植(zhi)(zhi)物應答缺(que)鎂(mei)脅(xie)迫存在一些獨(du)特的(de)(de)(de)信號(hao)通(tong)路(lu)，且與植(zhi)(zhi)物激素有關(guan)，而乙(yi)烯在應答缺(que)鎂(mei)過程中(zhong)發揮了(le)關(guan)鍵作用；缺(que)鎂(mei)還同步增強了(le)植(zhi)(zhi)物抗氧(yang)化(hua)酶(mei)(mei)活性(xing)。
表 1 鎂元(yuan)素缺(que)乏處理第 8 天擬南芥新成(cheng)熟(shu)葉片和根(gen)系的生理參數
DHA：ASC，氧(yang)化態脫氫抗壞(huai)血(xue)酸：抗壞(huai)血(xue)酸；GSSG : GSH，氧(yang)化型(xing)谷胱(guang)甘肽：谷胱(guang)甘肽；Ctrl，鎂元素(su)充(chong)足(zu)的植株；-Mg，鎂元素(su)缺乏的植株
Hermans, C., et al., Systems analysis of the responses to long-term magnesium deficiency and restoration in Arabidopsis thaliana. New Phytologist, 2010. 187(1): p. 132-144.
1.3 乙烯測(ce)定在病(bing)菌(jun)感染研(yan)究(jiu)中的應(ying)用舉(ju)例
實(shi)驗內容簡介：以(yi)(yi)品種為 Money Maker 和 Daniela 的(de)(de)(de)(de)(de)成(cheng)熟番(fan)(fan)(fan)(fan)茄(qie)(qie)果實(shi)為材料，研究了其(qi)感(gan)染(ran)番(fan)(fan)(fan)(fan)茄(qie)(qie)灰(hui)(hui)霉(mei)(mei)病(bing)菌株 VTF1 的(de)(de)(de)(de)(de)乙烯(xi)釋放(fang)(fang)。灰(hui)(hui)霉(mei)(mei)病(bing)菌可以(yi)(yi)在體外產(chan)生(sheng)乙烯(xi)，其(qi)乙烯(xi)釋放(fang)(fang)與其(qi)說(shuo)(shuo)與分(fen)(fen)生(sheng)孢(bao)子萌(meng)發相關，不如說(shuo)(shuo)與菌絲生(sheng)長更相關，且分(fen)(fen)生(sheng)孢(bao)子濃度(du)越(yue)大(da)真(zhen)菌的(de)(de)(de)(de)(de)乙烯(xi)釋放(fang)(fang)越(yue)多(duo)。感(gan)染(ran)灰(hui)(hui)霉(mei)(mei)病(bing)的(de)(de)(de)(de)(de)兩種番(fan)(fan)(fan)(fan)茄(qie)(qie)的(de)(de)(de)(de)(de)乙烯(xi)釋放(fang)(fang)規律與灰(hui)(hui)霉(mei)(mei)病(bing)菌類似；但釋放(fang)(fang)量(liang)是其(qi) 100 倍。結(jie)合(he)受感(gan)染(ran)番(fan)(fan)(fan)(fan)茄(qie)(qie)的(de)(de)(de)(de)(de)細(xi)胞學參數，研究結(jie)果表明(ming)：番(fan)(fan)(fan)(fan)茄(qie)(qie)-真(zhen)菌系統的(de)(de)(de)(de)(de)乙烯(xi)釋放(fang)(fang)不是由番(fan)(fan)(fan)(fan)茄(qie)(qie)灰(hui)(hui)霉(mei)(mei)病(bing)菌引起的(de)(de)(de)(de)(de)，雖說(shuo)(shuo)與其(qi)內部的(de)(de)(de)(de)(de)真(zhen)菌生(sheng)長速率十分(fen)(fen)同(tong)步(bu)。
圖 2 真菌（160 μl 懸浮液）的乙烯產量(liang)
● 1.5*108 灰霉病菌分生孢子 ml-1 ▲ 2*107 灰霉病菌分生孢子 ml-1 ■ 2*105 灰霉病菌分生孢子 ml-1
圖3 模擬(ni)感染和(he)不同(tong)濃度(du)番茄(qie)灰霉病菌(jun)感染的(de)兩種(zhong)番茄(qie)的(de)乙烯釋放(fang)
A.番(fan)茄品種 Money Maker；B.番(fan)茄品種 Daniela；
○ 模擬番茄灰霉病菌感染 ● 1.5*108 灰霉病菌分生孢子 ml-1 ▲ 2*107 灰霉病菌分生孢子 ml-1 ■ 2*105 灰霉病菌分生孢子 ml-1
Cristescu, S.M., et al., Ethylene Production by Botrytis cinerea In Vitro and in Tomatoes. Applied and Environmental Microbiology, 2002. 68 (11): p. 5342-5350.
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