蜂巢矩陣(zhen)葉綠素熒光成像系(xi)統(tong)
HEXAGON-IMAGING-PAM
葉綠素熒光(guang)成(cheng)像系統的“六邊形戰士(shi)”
精度高，面積大，功能全，應用廣(guang)，文獻(xian)多，數據可視(shi)化！
HEXAGON-IMAGING-PAM是德國WALZ公司新推出的大型蜂巢矩陣葉綠素熒光成像系統。它憑借高精度的脈沖振幅調制（PAM）技術，可以對20×24cm的區域進行成像。分辨率高達1.2 MP（1000 x 1200 px, 2x2 binning技術，實際是2000×2400），像素尺寸3.45 x 3.45 μm。
超高分辨率的(de)基礎是成像(xiang)區域光場的(de)均(jun)勻性，在設計過程中，光源(yuan)陣列中LED的位置是(shi)經過(guo)精心布局(ju)的，以保證測量(liang)區域內無陰影，所有成像(xiang)區域內的樣品均勻(yun)照(zhao)光，樣品間的差異(yi)可以盡收眼(yan)底(di)。大功(gong)率(lv)LED面板的冷(leng)卻效果(guo)非常好，可以大(da)限度的延長LED的使用壽(shou)命。
增加(jia)遠紅光（FR）LED 面(mian)板(ban)，可用于(yu)測量(liang)所研究(jiu)樣品的Fo'值(zhi)。
HEXAGON-IMAGING-PAM采用蜂巢矩陣式LED面(mian)板拼接技術，單個六邊形蜂巢矩陣單元(yuan)之間(jian)LED的(de)不平橫可以獨立補償，初(chu)衷是為實現樣品區(qu)域(yu)的(de)理想(xiang)照明提供更優的(de)選擇。
盡管成像(xiang)區(qu)域很大，但是它依(yi)然足夠靈活，可以測量各(ge)種類型的樣品，如盆栽植(zhi)物，穴(xue)盤中培(pei)養的植(zhi)物，培(pei)養皿(min)上的植(zhi)物或多孔板(ban)中的藻類懸(xuan)浮液。
滑動門設計(ji)，集成(cheng)安全(quan)關閉功能，開門狀態(tai)下，飽和脈沖(chong)的強(qiang)度(du)會被抑(yi)制以(yi)保(bao)護(hu)操作人員(yuan)的眼睛(jing)。
主要功能
l 原位測量：活體植物葉綠素熒(ying)光成像，直觀顯(xian)示樣(yang)品光合作用光能利用差異(yi)，可導出彩(cai)色(se)圖像。
l 成像功能(neng)：對Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、PS/50=ETR、Inh等(deng)參數進行成(cheng)像分析。測定調節性能量耗散(san)Y(NPQ)，反映植(zhi)物光保護能力，測定非(fei)調節性(xing)能量耗散Y(NO)，反(fan)映植物(wu)光損傷程度。
l 程(cheng)序測(ce)(ce)(ce)量(liang)功(gong)能(neng)：可自動(dong)程(cheng)序測(ce)(ce)(ce)量(liang)熒(ying)光誘導曲線、快速光曲線和暗弛豫，也可手動(dong)測(ce)(ce)(ce)量(liang)；在測(ce)(ce)(ce)量(liang)過程(cheng)中(zhong)能(neng)自動(dong)分析所有熒(ying)光參(can)數的變(bian)化趨勢(shi)；可以(yi)預編程(cheng)進(jin)行自定義實驗流程(cheng)，如模擬(ni)波動(dong)光。
l AOI功能：可在測量前或測量后任意選擇感興趣的區域（AOI），程序(xu)將自動(dong)對(dui)選(xuan)擇的AOI的數據進行變(bian)化趨勢分析，并在報告文件(jian)中顯示相關AOI的數據。所有報(bao)告文件中(zhong)顯示的數據都可導出到EXCEL文件中。
l 成(cheng)像異(yi)質性分析(xi)功能：對任意(yi)參數(shu)任意(yi)時間(jian)的(de)成(cheng)像，可(ke)在圖像上(shang)任意(yi)選取兩點(dian)，軟(ruan)件自(zi)動(dong)對兩點(dian)間(jian)的(de)數(shu)據進行橫向(xiang)異(yi)質性分析(xi)，并可(ke)導出到EXCEL文件中。
l 成像(xiang)數據(ju)范圍分析功能：對任(ren)意參(can)數任(ren)意時間的成像(xiang)，可分析任(ren)意兩個熒光數值之間有多少個像(xiang)素點，多少面積（cm2）。
l 突(tu)變株篩選功能：可跟據(ju)成像結果(guo)快速(su)篩選光合(he)、產氫/油、抗逆（抗鹽、抗旱、抗病等(deng)）等(deng)突變株。
l 微藻毒(du)理研究功(gong)能：可(ke)同時測量(liang)4塊96孔板，即384個微藻樣品（對(dui)(dui)照和(he)處理(li)組(zu)）的(de)光合活性，軟(ruan)件自動(dong)給出(chu)處理(li)組(zu)樣品相對(dui)(dui)于對(dui)(dui)照組(zu)的(de)光合抑(yi)制(zhi)百分比(bi)。
應用領域(yu)
l 光合作用研究：可以在完(wan)全相同的條件下同時對大量樣品(pin)進行成像
l 植物病(bing)理學：病(bing)斑(ban)部位（包括肉(rou)眼不可見時）成像以及病(bing)斑(ban)擴(kuo)散的時空動力(li)學
l 植物(wu)脅(xie)迫(po)生(sheng)理學：肉眼不(bu)可見(jian)生(sheng)物(wu)/非生(sheng)物(wu)脅迫損(sun)傷的早期檢測
l 遺傳育(yu)種：出苗(miao)后大規(gui)模快速(su)篩選高(gao)光(guang)合/抗旱/抗熱/抗凍/抗(kang)病(bing)等植(zhi)株
l 突(tu)變(bian)株(zhu)篩選：快(kuai)速篩選模式植物(wu)的光合突(tu)變(bian)株(zhu)、抗(kang)逆(ni)突(tu)變(bian)株(zhu)、產氫微藻突(tu)變(bian)株(zhu)等
l 微藻(zao)毒理學(xue)：不同毒物(wu)濃(nong)度(du)多(duo)個(ge)重復的樣品一(yi)次測(ce)完，軟件自動計算(suan)抑制比率
l 其它多種擴展研究
成像參(can)數(shu)
Fo, Fm, F, Ft, Fm', Fv/Fm, Y(II), qL, qP, qN, NPQ, Y(NPQ), Y(NO), PS/50=ETR,Inh.等
產地：德國WALZ
參考文獻
數據來源：光合作用文(wen)獻Endnote數據庫(ku)，原始數據來源：Google Scholar。
注：HEXAGON-IMAGING-PAM為新(xin)產(chan)品，暫無文獻發表，研究成果可參考M-IMAGING-PAM發表(biao)文(wen)章。
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