大腸桿菌便攜式檢測套件——IncuBactTM
IncuBact是利用Amiscience內(nei)置(zhi)加熱器的手持熒光(guang)計(ji)(ji)(ji),配置(zhi)大(da)(da)腸桿菌(jun)現場檢(jian)(jian)(jian)測(ce)試(shi)劑盒。將樣品(pin)制備(bei)在(zai)0.5ml的樣品(pin)管中(zhong),放(fang)置(zhi)在(zai)保持38℃的手持熒光(guang)計(ji)(ji)(ji)中(zhong)進(jin)行(xing)檢(jian)(jian)(jian)測(ce)。在(zai)培(pei)養(yang)(yang)過程中(zhong),大(da)(da)腸桿菌(jun)釋放(fang)與培(pei)養(yang)(yang)介(jie)質產生熒光(guang)信(xin)號(hao)的酶,通過熒光(guang)計(ji)(ji)(ji)的進(jin)行(xing)檢(jian)(jian)(jian)測(ce)。如果(guo)信(xin)號(hao)的增長達到(dao)預(yu)先設定的臨界(jie)值,即(ji)確認檢(jian)(jian)(jian)測(ce)陽性(xing)。由于熒光(guang)計(ji)(ji)(ji)的靈敏度(du)極高,單一的健康細菌(jun)可(ke)以在(zai)短至10小時的培(pei)養(yang)(yang)時間(jian)(jian)內(nei)被(bei)檢(jian)(jian)(jian)測(ce)到(dao)。如果(guo)大(da)(da)腸柑橘的數(shu)量較多(duo),可(ke)以在(zai)更短的時間(jian)(jian)內(nei)檢(jian)(jian)(jian)測(ce)到(dao)。就地檢(jian)(jian)(jian)測(ce)提供了(le)預(yu)警的可(ke)能。通過信(xin)號(hao)的增長的臨界(jie)時間(jian)(jian),還可(ke)以半定量地估計(ji)(ji)(ji)原始樣品(pin)中(zhong)健康大(da)(da)