主要功能
成像功能(neng):對 Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等至少 17 種參數(shu)進行成像分析。測(ce)(ce)定調節性能(neng)量(liang)(liang)耗散 Y(NPQ),反(fan)映植物(wu)光(guang)(guang)保護能(neng)力,測(ce)(ce)定非調節性能(neng)量(liang)(liang)耗散Y(NO),反(fan)映植物(wu)光(guang)(guang)損傷程度。
程(cheng)(cheng)序(xu)測(ce)量功能:可程(cheng)(cheng)序(xu)測(ce)量熒光誘(you)導曲(qu)線、快速光曲(qu)線和暗弛(chi)豫,也可手動(dong)(dong)測(ce)量;在測(ce)量過(guo)程(cheng)(cheng)中能自動(dong)(dong)分析所有熒光參數的變化趨勢
AOI 功能:可(ke)在(zai)測量前或測量后任意選(xuan)擇感(gan)興趣的(de)區域(AOI),程序將自動對選(xuan)擇的(de) AOI 的(de)數(shu)據進(jin)行變化趨勢分析,并在(zai)報告文(wen)件(jian)中(zhong)顯示相關 AOI 的(de)數(shu)據。所有報告文(wen)件(jian)中(zhong)顯示的(de)數(shu)據都可(ke)導出(chu)到 EXCEL 文(wen)件(jian)中(zhong)。
成(cheng)像異質性(xing)分(fen)析功能:對(dui)任意參數任意時間的(de)成(cheng)像,可(ke)在(zai)圖像上任意選取(qu)兩點(dian),軟(ruan)件(jian)自(zi)動對(dui)兩點(dian)間的(de)數據進(jin)行橫向異質性(xing)分(fen)析,并可(ke)導出到(dao) EXCEL 文件(jian)中。
成像數據范圍分析功能:對任意參數任意時間的成像,可分析任意兩個熒光數值之間有多少個像素點,多少面積(cm2)。
突變株篩選(xuan)功能:可跟據成像結果快(kuai)速篩選(xuan)光合、產氫(qing)/油、抗逆(抗鹽、抗旱(han)、抗病等(deng))等(deng)突變株。
微藻毒理研究(jiu)功能(neng):可同時測量(liang) 96 個微藻樣品(對照(zhao)和處理組)的光(guang)合活性(xing),軟(ruan)件自(zi)動給出處理組樣品相對于對照(zhao)組的光(guang)合抑制百(bai)分(fen)比。
吸(xi)光(guang)(guang)系數(shu)(shu)測(ce)量功(gong)能:快速測(ce)量葉(xie)片的(de)吸(xi)光(guang)(guang)系數(shu)(shu)。吸(xi)光(guang)(guang)系數(shu)(shu)測(ce)量光(guang)(guang)源: 16 個紅光(guang)(guang)(650 nm)和(he) 16 個近紅外(wai)(780 nm)LED,用(yong)于測(ce)量植(zhi)物葉(xie)片或藻類(lei)樣品(pin) PAR 吸(xi)光(guang)(guang)系數(shu)(shu)。
光強異質性:測量區域光強異質性≤±2%
測量參數
Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等。
應用領域
光合作用(yong)研究:可以(yi)在(zai)完全相同的條(tiao)件下同時(shi)對大量樣品進行(xing)成像
植物病理(li)學:病斑部位(包(bao)括肉(rou)眼不可見時(shi))成(cheng)像以及病斑擴散的時(shi)空動力學
植物脅(xie)迫生 理學:肉眼(yan)不(bu)可(ke)見脅(xie)迫損(sun)傷的早期檢(jian)測
遺傳育種:出(chu)苗后大規模(mo)快速篩選高光合/抗(kang)旱/抗(kang)熱/抗(kang)凍/抗(kang)病(bing)等植株
突(tu)變株篩選:快速篩選模式(shi)植物的(de)光(guang)合突(tu)變株、抗逆突(tu)變株、產氫微(wei)藻突(tu)變株等(deng)
微藻(zao)毒(du)理學:不同毒(du)物濃度多個重復的樣品一次(ci)測(ce)完,軟(ruan)件自(zi)動(dong)計算(suan)抑制比率
分(fen)子(zi)生物學:宏觀水(shui)平上(shang)檢測樣品的綠(lv)色熒光(guang)蛋白(bai)(GFP)熒光(guang)
其它多種擴展研究
選購指南
一、熒光成像 MAXI 版
系統組成:通(tong)用型主機,LED 供電單元, LED 光源(yuan),CCD,數據線,軟(ruan)件等。
注意:高等植物或真核藻類測量時選擇藍光光源(推薦),藍藻測量請選擇紅光光源。
熒光成(cheng)像(xiang) MAXI 版本(ben) |
二、熒光成像 MINI 版
系統組成:通用型主機(下圖中未顯(xian)示),LED 光源(如下圖),CCD,數(shu)據線,軟件等。
注意:高等植物或真核藻類測量時選擇藍光光源(推薦),藍藻測量請選擇紅(hong)光(guang)光源。
熒光成像 MINI 版 |
三、熒光成像 MICROSCOPY 版
系統組成:通用型主(zhu)機,特制顯微鏡,CCD,數據線,軟件等(deng)。
熒光(guang)成像 MICROSCOPY 版 |
葡萄葉片(pian) Y(NPQ) 成像 | 葡(pu)萄葉片 PS/50 成像結果 | MINI 版本成像結果 |
| | |
顯微版本成像結果 | 軟件成(cheng)像界面(mian) | 慢速誘(you)導(dao)動力學曲線(xian) |
產地:德國 WALZ
參考文獻
數據來源:光合作用文(wen)獻 Endnote 數(shu)據(ju)庫,更(geng)新(xin)至 2021 年(nian) 1 月,文(wen)獻數(shu)量(liang)超過 10000 篇(pian)
原始數據來源:Google Scholar
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